John Flynn

The Use of Preparative Supercritical Fluid Chromatography for the Isolation of Cannabidolic Acid (CBDA) From Cannabis Extracts

John J Flynn, Andrew J Aubin

Waters Corporation, MA, USA

Preparative supercritical fluid chromatography (SFC) has been used for purification applications in the pharmaceutical and natural products industries for many years.  One of the primary advantages of SFC is the low volume of organic solvent (co-solvent) required to complete the target isolation.  This allows for a much faster and more economical cleanup of the component of interest.  In addition, the co-solvent is often times a low hazard material such as methanol or ethanol, which can be easily removed from the collected product. One such application is the isolation of cannabidiolic acid (CBDA) from cannabis extracts.  CBDA is the precursor to the non-psychoactive cannabinoid, cannabidiol (CBD), known for several health benefits, including relief from pain, inflammation and seizures.  However, in order to useful, it must be isolated from the isomer tetrahydrocannabinolic acid (THCA), which when decarboxylated, becomes the psychoactive component tetrahydrocannabinol (THC).  This poster will present a method of isolating CBDA through the use of supercritical carbon dioxide, and will demonstrate method development and scale-up.